1. 學術科研

        我校揭示水產養殖中抗生素誘導的多重耐藥基因轉移模式

        發布日期:2022-11-09 發表者:陳治國 瀏覽次數:


           南湖新聞網訊(通訊員 劉通)近日,華中農業大學生命科學技術學院益生菌智造創新團隊聯合信息學院鄭金水副教授在抗性基因傳播方面的研究成果以“Enrofloxacin-induced transfer of multiple-antibiotic resistance genes and emergence of novel resistant bacteria in red swamp crayfish guts and pond sediments ”為題在Journal of Hazardous Materials上發表。本研究揭示了在抗生素壓力下抗性基因(ARGs)的選擇性擴增和傳遞以及ARGs與宿主細菌的相關性,并基于腸道和沉積物中的微生物群的宏基因組序列重建了新的多重耐藥細菌基因組。

           抗生素耐藥基因(ARGs)可以從環境微生物轉移到人類病原微生物,從而導致細菌感染治療失敗,特別是對多種抗生素具有耐藥性的致病菌感染是死亡率的重要原因。水產養殖系統因為與水、沉積物和其他生態系統有著廣泛的聯系,抗生素在其的應用為耐藥性的傳播提供了一個廣泛的環境途徑。然而,在抗生素暴露的水產養殖系統中,ARGs的來源、歷時變化和流動性仍然不清楚。本研究以小龍蝦養殖為模型,分析了人工給藥后(第三代喹諾酮類藥物恩諾沙星)(1)小龍蝦腸道和底泥各種ARG的時間變化,(2)小龍蝦腸道和沉積物中細菌之間ARG轉移的機制,(3)ARG與宿主細菌之間的相互作用,以確定耐藥基因偏愛的細菌。

           研究結果表明,恩諾沙星的應用不僅增加了喹諾酮類耐藥ARGs的相對豐度,而且促進了對其他抗生素耐藥ARGs的出現。此外,抗生素的應用誘導ARGs通過水平基因轉移(HGT)在小龍蝦腸道細菌和沉積物細菌中傳播。值得注意的是,在抗生素壓力下,轉座酶介導的重組是ARGs轉移的主要驅動因素。腸桿菌科可能是自然界中一個流動的ARG庫,匯集了不同來源的ARG。研究還表明,恩諾沙星的應用誘導了一些攜帶多種ARGs的新型抗生素耐藥物種。這些攜帶ARGs的細菌對水生動物和環境的污染值得高度關注。該研究強調了抗生素應用引起的ARG通過HGT在養殖環境中傳播的風險。研究結果可為抗生素耐藥性管理提供參考。

           生命科學技術學院博士研究生金雪霞為論文第一作者,彭楠教授、鄭金水教授為通訊作者。同時課題組劉思真、劉通等研究生參與了研究。本研究得到了湖北洪山實驗室重大項目和生科院龍運計劃等支持。

        【英文摘要】
        Antibiotic resistance genes (ARGs) can be transferred from environmental microbes to human pathogens, thus leading to bacterial infection treatment failures. The aquaculture polluted by over-used antibiotics is considered as a notorious reservoir of ARGs. However, the origin, diachronic changes, and mobility of ARGs under antibiotic exposure in aquaculture systems remain elusive. Our findings showed that enrofloxacin application also increased the relative abundance of various ARGs in addition to quinolone-resistance genes and induced ARG dissemination in crayfish gut and sediment bacteria. Further investigation indicated that the transposase-mediated recombination was the major driver of horizontal gene transfer (HGT) of ARGs under antibiotic stress. Notably, enrofloxacin application also induced the generation of some metagenome-assembled genomes (MAGs) carrying multiple ARGs, which were identified as novel species. Additionally, Enterobacteriaceae constituted a mobile ARG pool in aquaculture. Therefore, aquaculture provides potential wide environmental pathways for generation and spread of antibiotic resistance. Our findings of ARG temporal variations and dissemination pattern in aquaculture with artificial use of antibiotics are critical to the management of antibiotic resistance, which is of great ecosystem and health implications.

        論文連接:https://doi.org/10.1016/j.jhazmat.2022.130261

        審核人:彭楠

        原文鏈接:http://news.hzau.edu.cn/2022/1109/65050.shtml

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